Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice

Authors

Anatol Manaenko, Department of Physiology and Pharmacology, Loma Linda University Medical Center, Loma Linda, CA, USA.Follow
Tim Lekic, Department of Physiology and Pharmacology, Loma Linda University Medical Center, Loma Linda, CA, USA.
Qingyi Ma, Department of Physiology and Pharmacology, Loma Linda University Medical Center, Loma Linda, CA, USA.Follow
John H. Zhang, Department of Physiology and Pharmacology, Loma Linda University Medical Center, Loma Linda, CA, USA.Follow
Jiping Tang, Department of Physiology and Pharmacology, Loma Linda University Medical Center, Loma Linda, CA, USA.Follow

Document Type

Article

Publication Date

5-1-2013

Publication Title

Critical care medicine

E-ISSN

1530-0293

Abstract

OBJECTIVE: Hydrogen inhalation was neuroprotective in several brain injury models. Its mechanisms are believed to be related to antioxidative stress. We investigated the potential neurovascular protective effect of hydrogen inhalation especially effect on mast cell activation in a mouse model of intracerebral hemorrhage. DESIGN: Controlled in vivo laboratory study. SETTING: Animal research laboratory. SUBJECTS: One hundred seventy-one 8-week-old male CD-1 mice were used. INTERVENTIONS: Collagenase-induced intracerebral hemorrhage model in 8-week-old male CD-1 mice was used. Hydrogen was administrated via spontaneous inhalation. The blood-brain barrier permeability and neurologic deficits were investigated at 24 and 72 hours after intracerebral hemorrhage. Mast cell activation was evaluated by Western blot and immuno-staining. The effects of hydrogen inhalation on mast cell activation were confirmed in an autologous blood injection model intracerebral hemorrhage. MEASUREMENT AND MAIN RESULTS: At 24 and 72 hours post intracerebral hemorrhage, animals showed blood-brain barrier disruption, brain edema, and neurologic deficits, accompanied with phosphorylation of Lyn kinase and release of tryptase, indicating mast cell activation. Hydrogen treatment diminished phosphorylation of Lyn kinase and release of tryptase, decreased accumulation and degranulation of mast cells, attenuated blood-brain barrier disruption, and improved neurobehavioral function. CONCLUSION: Activation of mast cells following intracerebral hemorrhage contributed to increase of blood-brain barrier permeability and brain edema. Hydrogen inhalation preserved blood-brain barrier disruption by prevention of mast cell activation after intracerebral hemorrhage.

Volume

41

Issue

5

First Page

1266

Last Page

75

DOI

10.1097/CCM.0b013e31827711c9

PubMed ID

23388512

Recommended Citation

Manaenko, Anatol; Lekic, Tim; Ma, Qingyi; Zhang, John H.; and Tang, Jiping, "Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice" (2013). Loma Linda University Faculty Publications. 359.
https://scholarsrepository.llu.edu/fac_pubs/359