Abstract

Interleukin-10 (IL-10) is a cytokine with anti-inflammatory activities. IL-10 knockout (KO) mice exhibit severe intestinal inflammation in the presence of normal gut flora. We hypothesized that IL-10 KO mice would also be susceptible to inflammatory periodontal disease resulting in alveolar bone loss (ABL). The purpose of this study was to compare naturally occurring ABL in IL-10 KO and wild type (WT) mice. Twelve IL-10 KO and 12 WT mice, age-(7 months) and sex-(male) matched, were used. Half of the mice were on 129/SvEv and half on C57BL/6J background. Animal heads were defleshed and treated to remove all organic material. ABL was measured as exposed molar root surface area (mm2) on the lingual aspect of mandibular quadrants. Blinded measurements were performed using a computer-assisted image analysis system. Animal ABL was the mean of the 2 quadrants. Group mean differences were analyzed by Mann-Whitney U test.

Mean (± sd; n = 12) ABL for the IL-10 KO and WT groups was 1.15 ± 0.32 and 0.75 ±0.15 mm2, respectively, a statistically significant difference (p = 0.0004). This difference between IL-10 KO and WT mice remained significant for each strain (129/SvEv; p = 0.007; C57BL/6J: p = 0.011). No significant difference in ABL between the 2 strains was found when all mice were analyzed together. There was a significant difference in ABL between 129/SvEv and C57BL/6J strains when WT mice were analyzed alone (p = 0.011), but not when IL-10 KO mice were analyzed.

The results of this study indicate that IL-10 KO mice are susceptible to accelerated alveolar bone loss, and suggest that IL-10 KO mice might be a useful model for the study of alveolar bone loss pathogenesis, including investigations of the reported association between chronic bowel inflammation and alveolar bone loss.

LLU Discipline

Periodontics

Department

Periodontics

School

Graduate School

First Advisor

Robert Kiger

Second Advisor

Hansel Fletcher

Third Advisor

Dimitris Tatakis

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2002

Date (Title Page)

8-2002

Language

English

Library of Congress/MESH Subject Headings

Interleukim-10; Alveolar Bone Loss; Periodontal Diseases -- pathology; Dental Plaque -- pathology

Type

Thesis

Page Count

x; 40

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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