Abstract

The purpose of this study was to identify the early hard tissue formed in experimental pulp exposures capped with calcium hydroxide, mineral trioxide aggregate (MTA) or bone morphogenetic protein-7 (BMP-7) using dentin sialoprotein (DSP) as a marker. The pulps of eighty-five maxillary first, second and third molar teeth from twenty male rats were experimentally exposed. The exposed pulps were capped with calcium hydroxide followed by restoration with amalgam (n = 20), with MTA alone as a pulp capping agent and final restoration (n = 20) or with BMP-7 followed by restoration with MTA (n = 20). Ten teeth with Class 1 occlusal preparations, no exposure and no restoration served as positive controls. Ten teeth that received pulp exposures and no restoration served as negative controls. Five untreated third molars served as additional controls. The animals were sacrificed at two and eight weeks. The specimens were prepared and evaluated histologically and with immunohistochemistry utilizing polycolonal antibodies raised against rat dentin sialoprotein (DSP). Pulps capped with calcium hydroxide demonstrated irregular hard tissue formation that did not stain for DSP. Pulps capped with MTA formed hard tissue that demonstrated significantly more immunostaining for DSP when compared to calcium hydroxide and BMP-7 (p = 0.0001 and p = 0.0031 respectfully). MTA capped pulps also demonstrated significantly more complete bridging when compared to calcium hydroxide and BMP-7 (p = 0.0008 and p = 0.008 respectfully). Pulps capped with BMP-7 demonstrated a hard tissue that was bone-like in appearance and devoid of DSP staining.

Department

Dentistry

School

Graduate School

First Advisor

Mahmoud Torabinejad

Second Advisor

Shahrokh Shabahang

Third Advisor

Kenneth Wright

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2002

Date (Title Page)

8-2002

Language

English

Library of Congress/MESH Subject Headings

Dental Pulp Capping; Calcium Hydroxide; Dentin Bonding Agents; Sialoglycoproteins

Type

Thesis

Page Count

x; 57

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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