Abstract

The purpose of the experiment was to compare the development of Trichinella spiralis in germ-free, Lactobacillus casei monocontaminated, Escherichia coli monocontaminated and conventional mice. The criteria used in the experiments were the recovery of adults and larvae of T. spiralis after 72 hours and one month post-infection, respectively. which gave an indication of the effects of no bacteria or only a certain, single species of bacterium on the development of this nematode in mice.

Germ-free T. spiralis larvae were obtained by washing them several times with sterile normal saline, and treatment with antibiotics. The gavaging procedure was done without anesthetization on the first two series of experiments, but on the last series diluted sodium nembutal was used before the gavaging to introduce germ-free sterile larvae since this procedure had to be done in the germ-free isolators.

The number of adults and larvae of T. spiralis recovered was higher in conventional mice than in the rest of the groups of mice. The number of T. spiralis larvae was approximately twice as great in conventional mice as compared with L. casei monocontaminated, E. coli monocontaminated and germ-free mice.

Experiments were carried, out to find the location of monocontaminants established in the alimentary tract. These showed that both monocontaminants established in the alimentary tract. They showed that both monocontaminants were established in both small and large intestine. The number of bacteria was not determined, but qualitative evaluation showed more bacteria were located in the large intestine than in the small intestine. It is concluded from the results of the experiment that bacteria are needed for optimum development of T. spiralis in its host.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate School

First Advisor

Edward D. Wagner

Second Advisor

H.S. Lau

Third Advisor

R. Bruce Wilcox

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1970

Date (Title Page)

8-1970

Language

English

Library of Congress/MESH Subject Headings

Trichinella

Type

Thesis

Page Count

vi; 43

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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