An In Vivo Perfusion Apparatus for Studies on Gingival Disease in the Albino Rat : Assessment by a Perfusion of the Enzymes Collagenase and Hyaluronidase

Author

James A. Smith

Abstract

This thesis describes an in vivo perfusion apparatus which can continuously deliver controlled quantities of solutions to localized, contralateral intraoral areas in albino rats. Three animals were per fused on the experimental area (the mesial sulcular area of the maxillary first molar) on one side with tap water and Trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. Solutions of .5%, 1%, and 2% hyaluronidase and collagenase were perfused on the experimental areas of nine animals. When both enzymes were used, hyaluronidase always preceded collagenase in sequence. Rate of flow ranged from 4.8 to 7.1 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data obtained from animals perfused with collagenase for periods over sixty hours at from 1% to 2% concentration indicated moderate to severe inflammatory changes on the experimental side. More meaningful results should be obtained with the modifications discussed in this report

LLU Discipline

Periodontics

Department

Dentistry

School

Graduate School

First Advisor

Richard C. Oliver

Second Advisor

Edwin M. Collins

Third Advisor

G. Conrad Hornbuckle

Fourth Advisor

Eugene Rathbun

Fifth Advisor

John DeVincenzo

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1970

Date (Title Page)

6-1970

Language

English

Library of Congress/MESH Subject Headings

Gingival Diseases; Dental Equipment

Type

Thesis

Page Count

vii; 59

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Copyright

Author

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Recommended Citation

Smith, James A., "An In Vivo Perfusion Apparatus for Studies on Gingival Disease in the Albino Rat : Assessment by a Perfusion of the Enzymes Collagenase and Hyaluronidase" (1970). Loma Linda University Electronic Theses, Dissertations & Projects. 1625.
https://scholarsrepository.llu.edu/etd/1625

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives