Abstract

Complementarity studies were conducted between bacteriophage P4/2 DNA and the DNAs of its naturally lysogenic host Salmonella potsdam strain 4 (SP4) and its sensitive host Salmonella potsdam strain 2 (SP2). Calf thymus DNA was used as a control.

Nitrocellulose membranes were used to adsorb varying concentrations of unlabeled DNA, using P4/2 DNA, SP4 DNA, SP2 DNA, and calf thymus DNA. A constant amount of P32 -labeled P4/2 DNA was added to each membrane and the hybrids were allowed to form. After thorough rinsing to get rid of unbound labeled DNA, radioactivity of the samples was read in a liquid scintillation spectrometer and the percentages of complementarity were calculated.

Approximately eleven and one-half times more complementarity was found to exist between P4/2 DNA and that of its naturally lysogenic host than between P4/2 DNA and that of its sensitive host. This difference may be due to the presence of the P4 prophage on the lysogenic SP4 genome and its absence in the non-lysogenic host SP2 genome.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate School

First Advisor

Robert L. Nutter

Second Advisor

Leonard R. Bullas

Third Advisor

U. D. Register

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1968

Date (Title Page)

8-1968

Language

English

Library of Congress/MESH Subject Headings

Bacteriophages; Salmonella; DNA

Type

Thesis

Page Count

vii; 43

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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