Abstract

Cell-mediated immunity was investigated in two BALB/c mouse tumor systems using the lymphocyte transformation test with phytohemagglutinin as the mitogen. This lymphoproliferative response was quantitated using the Stimulation Index (SI). Spleen cells from mice injected with #51 cells, a chemically-transformed colon cell line, produced a SI value equal to that of normal mice. There was no evidence for suppressor cell activity in cell mixing experiments in which spleen cells from #51 cell-injected mice were mixed with spleen cells from normal mice. Following macrophage removal by Sephadex G-10 columns an d carbonyl iron ingestion, there were no significant changes in the SI values for spleen cells from the #51 cell-injected mice. In contrast, spleen cells from mice injected with H238 cells, a herpes virus-transformed cell line, had a significantly lower SI value than that of normal mice. Suppressor cell activity was demonstrated in cell mixing experiments in which spleen cells from H238 cell-injected mice were mixed with normal spleen cells. Removal of adherent cells from spleen cells from H238 cell-injected mice by Sephadex G-10 columns restored the SI value to that of normal mice. An increased SI value was also seen after removal of phagocytic cells by carbonyl iron. These results suggested that cells with the functional properties of macrophages played an important part in the immunosuppression observed in the H238 tumor system. Comparison of the two macrophage depletion methods suggested that another cell population was also involved in the suppressive effect.

LLU Discipline

Physiology

Department

Physiology

School

Graduate School

First Advisor

Robert L. Nutter

Second Advisor

Robert W. Teel

Third Advisor

David A. Hessinger

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1983

Date (Title Page)

3-1983

Language

English

Library of Congress/MESH Subject Headings

Cell Migration Inhibition; Lymphoproliferative Disorders; Immunity, Cellular

Type

Thesis

Page Count

ill; 33

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

Download

Included in

Physiology Commons

Share

COinS