Abstract
Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells are clearly stained using the immuno-peroxidase procedure, whereas they do not stain with the conventional tetrazolium method for LDH.
LLU Discipline
Anatomy
Department
Anatomy
School
Graduate School
First Advisor
Paul J. McMillan
Second Advisor
William M. Hooker
Third Advisor
Donald D. Rafuse
Degree Name
Master of Science (MS)
Degree Level
M.S.
Year Degree Awarded
1976
Date (Title Page)
6-1976
Language
English
Library of Congress/MESH Subject Headings
Lactate Dehydrogenase
Type
Thesis
Page Count
ill; 20
Digital Format
Digital Publisher
Loma Linda University Libraries
Copyright
Author
Usage Rights
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Recommended Citation
Bustos-Arango, Luis M., "Immuno-histochemical method for demonstration of Lactic Dehydrogenase "M" subunits in Rat Skeletal Muscle, Kidney and Peripheral Nerve" (1976). Loma Linda University Electronic Theses, Dissertations & Projects. 645.
https://scholarsrepository.llu.edu/etd/645
Collection
Loma Linda University Electronic Theses and Dissertations
Collection Website
http://scholarsrepository.llu.edu/etd/
Repository
Loma Linda University. Del E. Webb Memorial Library. University Archives