Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells are clearly stained using the immuno-peroxidase procedure, whereas they do not stain with the conventional tetrazolium method for LDH.
Paul J. McMillan
William M. Hooker
Donald D. Rafuse
Master of Science (MS)
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This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Bustos-Arango, Luis M., "Immuno-histochemical method for demonstration of Lactic Dehydrogenase "M" subunits in Rat Skeletal Muscle, Kidney and Peripheral Nerve" (1976). Loma Linda University Electronic Theses, Dissertations & Projects. 645.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives